by icia icia

Objectives:
The purpose of this project: (i) a comprehensive study and development of new methods for rapid and sensitive analysis in order to determine estrogenic contaminants and heavy metals in food chain, at nanoscale, by using modern chromatographic and spectrometric techniques. It will be develop new analysis techniques for: PCB congeners, PAH, DDT, DDE, Dieldrin, phthalic esters, heavy metals;

OBJECTIVES The purpose of this project: (i) a comprehensive study and development of new methods for rapid and sensitive analysis in order to determine estrogenic contaminants and heavy metals in food chain, at nanoscale, by using modern chromatographic and spectrometric techniques. It will be develop new analysis techniques for: PCB congeners, PAH, DDT, DDE, Dieldrin, phthalic esters, heavy metals; (ii) validation of developed methods: experiments in order to optimize and establish the performance parameters for determination method of estrogenic contaminants – chromatographic separation and identification of organic species and heavy metals – detection limit, sensibility, selectivity, justness, fidelity, uncertainty, degree of recovery in accordance with RENAR, EURACHEM and European Co–operation for Accreditation.

The proposal is based on:

  • the institution infrastructure that allows the elaboration of advanced techniques to determine different organic compounds and heavy metals in environment samples at nanoscale; the institution has the required infrastructure to achieve the best conditions of a such project: Gas chromatography coupled mass spectrometry (GC-MS), High liquid chromatography coupled mass spectroscopy (LC-MS-MS), Gas chromatography with electron capture detection (GC-ECD), Gas chromatography with flame ionization detector (GC-FID), Inductively coupled plasma mass spectrometry (ICP-MS), Inductively coupled plasma atomic emission spectroscopy (ICP-AES);
  • research team’s experience in national and international projects for the assessment of environmental factors quality.

Project objectives:

  • development of new extraction methods of some estrogenic contaminants in order to analysis them with improved performance parameters;
  • mineralization above spectral analysis in order to determine heavy metals, in trace and ultra-trace, by microwave digestion in closed vessels, due to its significant superiority over classical mineralization (dry or wet in open vessels);
  • determination of heavy elements, in trace and ultra-trace concentration, by multielemental analysis due to the performance and capacity of ICP MS instrument in order to harmonize the Romanian legislation in the field of environmental and food quality assurance to international legislation, with emphasis on EU legislation.
DESCRIPTION Romanian’s integration in the European Union imposes a number of sustained actions in order to align with EU norms and legislation, on medium and long term, in the field of environment protection and quality improvement. The proposed project wants to be an answer to these actions by the elaboration of new methods, for determining some compounds with estrogenic activities and heavy metals in the environment.
A current problem in all the industrialized countries is the balance between the need for economic and social growth and the protection and quality improvement of the environment. Any kind of pollution represents an important danger with negative consequences on the environment and consequently on the human beings and their life. Alongside water and air, food is an important environment factor for ensuring the necessary substances for the good deployment of all the vital processes. Contaminants end up in food in a natural way, as a result of the human activities (agriculture, industrial emissions or cars), during cooking/processing and storage or can be added directly.
The organisms are often exposed to a complex mixture of pollutants, which include polychlorinated biphenyls (PCB), polychlorinated dibenzodioxins (PCDD), polychlorinated dibenzofurans (PCDF), poly-aromatic hydrocarbons (PAH), pesticides and metals. Pollutants that bioaccumulate in body may cause effects at cellular and molecular level resulting adverse effects, which cause changes at the level of the populations and communities in the future. Heavy metals are found in different concentration in the soil, water, air and food depending on the factors that determine their pollution.Opportunity of proposal results from:

  • Necessity to elaborate methods for the determination of organic contaminants and heavy metals; these methods will be available for decision-makers that will scientific base sustainable environmental management plans for registration in the EU environmental requirements;
  • Modern infrastructure necessary for the successful implementation of project; the laboratory LAM-ICIA is accredited in agreement with the request of the SR EN ISO/CEI 17025:2005;
  • Necessity to stimulate high performance scientific activity in the top areas of analytical chemistry, ecology, biochemistry, organic chemistry and IT with direct application in environmental protection. Further, a research group will be formed which will provide human resources with scientific research performance;
  • Exploitation of a validated investigational potential and recognized competence at European level (by involvement in several international projects) of project research team;
  • Establishing contacts to build international partnerships and participation in European projects;
  • Training young researchers (Master, Ph.D.), that will be involved in all project stages and will participate both at the experimentation of elaborated methods and the analysis of obtained results and database elaboration.
RESULTS ESTIMATED
  • Performed, complex, sensible and precise methods for the determination of estrogenic contaminants and heavy metals in food chain;
  • Database;
  • Patents proposal;
  • Articles published in ISI national and international journals in order to make better known the Romanian activity and scientific potential;
  • Communications;
  • Increasing the international recognition level of scientific, technical and technological development phase of the Romania.
RESULTS OBTAINED Phase 1

Phase 2

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Phase 8

Phase 9

 
Other Informations Published articles:

  1. Mirela Miclean, Oana Cadar, Cecilia Roman, Claudiu Tanaselia, Lucrina Stefanescu, Codruţa Ileana Stezar, Ioan Ştefan Groza, The influence of environmental contamination on heavy metals and organochlorine compounds levels in milk, Environmental Engineering and Management Journal, 2011, 10(1), 37-42. http://omicron.ch.tuiasi.ro/EEMJ/pdfs/vol10/no1/8_22_Miclean_11.pdf
  2. Mirela Miclean, Oana Cadar, Claudiu Tanaselia, Adriana Gog, Marin Senila, Ioan Stefan Groza, Levels of metals and organochlorine compounds in seafood consumed in Cluj-Napoca, Romania, Environmental Engineering and Management Journal, 2012, 11(1), 133-140. http://omicron.ch.tuiasi.ro/EEMJ/pdfs/vol11/no1/18_659_Miclean_11.pdf
  3. Mirela Miclean, Oana Cadar, Cecilia Roman, Determination of phthalates in bottled milk using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry, Studia Universitatis Babeș-Bolyai, Chemia, 2012, 57(1), 127-134. http://www.studia.ubbcluj.ro/arhiva/abstract.php?editie=CHEMIA&nr=1&an=2012&id_art=10415
  4. Dorina Simedru, Anca Naghiu, Oana Cadar, LC/MS/MS method for investigation of five usual pesticides from water, Studia Universitatis Babeș-Bolyai, Chemia, 2012, 57(1), 135-144. http://www.studia.ubbcluj.ro/arhiva/abstract.php?editie=CHEMIA&nr=1&an=2012&id_art=10416
  5. Claudiu Tanaselia, Mirela Miclean, Cecilia Roman, David Pop, An assessment of rare earth elements composition of Romanian meteoritic material using an inductively coupled plasma mass spectrometry method, Studia Universitatis Babeș-Bolyai, Chemia, 2012, 57(1), 145-150. http://www.studia.ubbcluj.ro/arhiva/abstract.php?editie=CHEMIA&nr=1&an=2012&id_art=10417

 
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